The 1st model animal experiment workshop was held.

In charge : Systems Neuroscience Group A03-1 Ozaki(A03-1 Group Co-I) and Masumizu(A03-1 Group PI)
Participants : Hosaka(A01-1 Group Co-I) and Kato(A01-1 Group Co-I), Lee(A01-2 Matsui Group)
Date : September 26th, 2024, 13:00-18:00 (Reception: 19:00-22:00)

　On September 26th, we held the 1st Model Animal Experiment Workshop. At the beginning of the workshop, Ozaki used slides to explain the surgical techniques for rodents (mouse). Specifically, he explained the series of procedures from headplate installation to adenovirus vector injection, using video clips. After that, he explained the outline of the two-photon microscope system and high-speed confocal microscope system in the laboratory, and acquired data using actual animals.

　Next, we explained the features and usage of “suite2p”, the analysis software for fluorescent calcium imaging. In the second half of the workshop, each participant analyzed the acquired data using their own laptop, and we also shared information about convenient ways to use suite2p, such as JupyterLab and Google Colab. The workshop was very meaningful, with active information exchange taking place throughout.

<Observing the headplate used in the surgical technique explained in the video>

<Explanation of the two-photon microscope system and simultaneous two-color fluorescence calcium imaging of neuronal fibers and cerebral cortex>

<Explanation of the high-speed confocal microscope system and fluorescence calcium imaging of the cerebral cortex during whisker sensory stimulation>

<Social gathering>

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